Pre-prints

  1. Milshteyn D, Winnikoff JR, Kocharian E, Armando AM, Dennis EA, Girguis PR, and Budin I. Homeostatic regulation of intrinsic lipid curvature in eukaryotic cells. bioRxiv 10.1101/2025.10.13.682232 (2025). Paper.
  2. Kim H, Lipp N-F, Juarez-Contreras I, Wong AM, and Budin I. Transport of sphingolipids by yeast Npc2 supports phase separation of the vacuole membrane. bioRxiv 10.1101/2025.09.14.676161 (2025). Paper.
  3. Lipp N-F and Budin I. An origin for a eukaryotic lipid transfer protein fold in Asgard archaea. bioRxiv 10.1101/2025.05.16.653879 (2025). Paper.
  4. Tsai Y-T, Lipp N-F, Seidel O, Varma R, Laguerre A, Solorio-Kirpichyan K, Wong A, Brea RJ, McGregor GH, Cordes T, Devaraj NK, Kuerschner L, Neal S, Metallo CM, and Budin I. 1-Deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathway. bioRxiv 10.1101/2025.05.13.652513 (2025). Paper.

2025

  1. Moore WM and Budin I. Imaging intracellular lipid heterogeneity with fluorogen-activating coincidence encounter sensing. Methods in Enzymology 10.1016/bs.mie.2025.11.015 (2025). Paper.
  2. Winnikoff JR and Budin I. Homeocurvature: A new dimension of membrane adaptation to extreme environments. Progress in Lipid Research 10.1016/j.plipres.2025.101355 (2025). Paper.
  3. Moore WM, Brea RJ, Knittel C, Wrightsman E, Hui B, Loui J, Ancajas CF, Best MD, Obara CJ, Devaraj NK, and Budin I. Leaflet-specific phospholipid imaging using genetically encoded proximity sensors. Nature Chemical Biology 10.1038/s41589-025-02021-z (2025). Paper.
    Featured in: Nature Chemical Biology, UCSD Today
  4. Lee CT, Venkatraman K, Budin I, and Rangamani P. Local enrichment of cardiolipin to transient membrane undulations. Biophysical Journal 124: 2476-2487 (2025) Paper.
  5. Juarez-Contreras I, Lopes LJS, Holt J, Yu-Liao L, O’Shea K, Ruiz-Ruiz J, Sodt A, and Budin I. Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation. Science Advances 11, eadu7190 (2025) Paper.
    Featured in: UCSD Today
  6. Venkatraman K, Lipp N-F, and Budin I. Origin and evolution of mitochondrial inner membrane composition. Journal of Cell Science 138 (9): jcs263780 (2025). Paper.

2024

  1. Venkatraman K, Lee CT, and Budin I. Setting the curve: the biophysical properties of lipids in mitochondrial form and function. Journal of Lipid Research 100643 (2024). Paper.
  2. Wong AM and Budin I. Organelle-targeted Laurdans measure heterogeneity in subcellular membranes and their responses to saturated lipid stress. ACS Chemical Biology 10.1021/acschembio.4c00249 (2024). Paper.
  3. Venkatraman K and Budin I. Cardiolipin remodeling maintains the inner mitochondrial membrane in cells with saturated lipidomes. Journal of Lipid Research 65: 100601 (2024). Paper.
  4. Winnikoff JR, Milshteyn D, Vargas-Urbano SJ, Perdaza MA, Armando AM, Quehenberger O, Sodt A, Gillilan RE, Dennis EA, Lyman E, Haddock SHD, and Budin I. Homeocurvature adaptation of phospholipids to pressure in deep-sea invertebrates. Science 384: 1482-1488 (2024). Paper.
    Featured in: Quanta Magazine, KPBS, Scientific American, Nature News, News from Science, Popular Science, Physics Today
  5. Kim H, Juarez-Contreras I, and Budin I. Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells. Methods in Enzymology 700: A, Chapter 4 10.1016/bs.mie.2024.02.015 (2024). Paper.

2023

  1. Kim H and Budin I. Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole. Journal of Biological Chemistry 10.1016/j.jbc.2023.105496 (2023). Paper.
  2. Chen J, Brea R, Fracassi A, Cho C, Wong A, Salvador-Castell M, Sinha S, Budin I, Devaraj NK. Rapid formation of non‐canonical phospholipid membranes by chemoselective amide‐forming ligations with hydroxylamines. Angewandte Chemie e202311635 (2023). Paper.
  3. Venkatraman K, Lee CT, Garcia GC, Mahapatra A, Perkins G, Kim K-Y, Pasolli HA, Phan S, Lippincott-Schwartz J, Ellisman M, Rangamani R, and Budin I. Cristae formation is a mechanical buckling event controlled by the inner mitochondrial membrane lipidome. The EMBO Journal (2023) e114054. Paper.

2021

  1. Winnikoff JR, Haddock SHD, and Budin I. Depth- and temperature-specific fatty acid adaptations in ctenophores from extreme habitats. Journal of Experimental Biology 224 (21): jeb242800 (2021). Paper.
  2. Moore WM, Milshteyn D, Tsai Y-T and Budin I. Engineering the bilayer: emerging genetic tool kits for mechanistic lipid biology. Current Opinion in Chemical Biology 65: 66-73 (2021). Paper.
  3. Seo AY, Sarkleti F, Budin I, Chang C-L, King CR, Kohlwein S-D, Sengupta P and Lippincott-Schwartz J. Vacuole phase-partitioning boosts mitochondria activity and cell lifespan through an inter-organelle lipid pipeline. bioRxiv 10.1101/2021.04.11.439383 (2021).

2020

  1. Nirody JA, Budin I and Rangamani P. ATP synthase: evolution, energetics, and membrane interactions. Journal of General Physiology 152: e201912475 (2020). Paper.
  2. Tsai Y-T, Moore WD, Kim H and Budin I. Bringing rafts to life: lessons learned from lipid organization across diverse biological membranes. Chemistry and Physics of Lipids 233: 104984 (2020).
  3. Skowronska-Krawczyk D and Budin I. Aging membranes: Unexplored functions for lipids in the lifespan of the central nervous system. Experimental Gerontology 131: 110817 (2020). Paper.
  4. Budin I, ARR Apel, NFC Hummel, A Mukhopadhyay and Keasling JD. Methods for mitochondria and organelle genome editing. US Patent App. 16/417,499 (2020).

2019

  1. Brenac L, Baidoo EEK, Keasling JD, and Budin I. Distinct functional roles for hopanoid composition in the chemical tolerance of Zymomonas mobilis. Molecular Microbiology 112:1564-1575 (2019). Paper.
  2. Jessop‐Fabre MM, Dahlin J, Biron MB, Stovicek V, Ebert BE, Blank LM, Budin I, Keasling JD, and Borodina I. The transcriptome and flux profiling of crabtree‐negative hydroxy acid‐producing strains of Saccharomyces cerevisiae reveals changes in the central carbon metabolism. Biotechnology Journal 14: 1900013 (2019). Paper.
  3. Degreif D, Cucu B, Budin I, Thiel G, and Bertl A. Lipid determinants of endocytosis and exocytosis in budding yeast. BBA – Molecular and Cell Biology of Lipids 1864: 1005-1016 (2019). Paper.
  4. Budin I and Keasling JD. Synthetic biology for fundamental biochemical discovery. Biochemistry 58:1464–1469 (2019). Paper.

2018

  1. Budin I, de Rond T, Chan Y, Chen L, Petzold C, and Keasling JD. Viscous control of cellular respiration by membrane lipid composition. Science 362:1186-1189 (2018). Paper. SM.
    • Featured in: Schon EA. Bioenergetics through thick and thin. Science 362:1114-1115 (2018).

2017

  1. Degreif D, de Rond T, Bertl, A, Keasling JD, and Budin I. Lipid engineering reveals regulatory roles for membrane fluidity in yeast flocculation and oxygen-limited growth. Metabolic Engineering 41:46-56 (2017). Paper.

2009-2016

  1. Budin I, Prywes N, Zhang N, and Szostak JW. Chain-length heterogeneity allows for the assembly of fatty acid vesicles in dilute solutions. Biophysical Journal 107:1582-90 (2014).
  2. Zhu TF, Budin I, and Szostak JW. Preparation of fatty acid micelles. Methods in Enzymology 533:283-8 (2013).
  3. Zhu TF, Budin I, and Szostak JW. Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder. Methods in Enzymology 533:275-82 (2013).
  4. Zhu TF, Budin I, and Szostak JW. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration. Methods in Enzymology 533:267-74 (2013).
  5. Budin I, Debnath A, and Szostak JW. Concentration-driven growth of model protocell membranes. Journal of the American Chemical Society 134:20812-9 (2012).
  6. Budin I and Devaraj NK. Membrane assembly driven by a biomimetic coupling reaction. Journal of the American Chemical Society 134:751-3 (2012).
  7. Budin I and Szostak JW. Physical effects underlying the transition from primitive to modern cell membranes. Proceedings of the National Academy of Sciences USA 108:5249-54 (2011).
  8. Budin I and Szostak JW. Expanding roles for diverse physical phenomena during the origin of life. Annual Reviews of Biophysics 39:245-63 (2010).
  9. Budin I, Bruckner RJ and Szostak JW. Formation of protocell-like vesicles in a thermal diffusion column. Journal of the American Chemical Society 131:9628-9 (2009).

    10.